Dear all,

this is my first question here!

following problem:

I have data on a certain complex association network (from monomers 1 ... 8 to complexes of all combinations, such as, 12 ... 13 ... 18 and so on until 12345678, so that I know

the structure of the network (255 vertices) the edges of the network the concentrations of the vertices From this data I now wish to infer the preferred route(s) of assembly for those complexes - which should be the rate constants for the edges, such as 1 --> 12 or 145 --> 1458. Does that make sense? Final result should be a set of paths of length 8 starting from (1..8) ending up at (12345678).

I have now tried various standalone applications for network creation/analysis as well as R packages. Metabolic flux analysis seemed promising for me, but it turned out that it only works with set external concentrations and assumes steady-state for all metabolites within the network. Which is not the case for me. Do you know of any alternative method?

Thanks and best wishes,

Simon

*Simon Prisner*

*PhD student*

*Humboldt-Universität zu Berlin / Max-Delbrück-Centrum Berlin*

*AG Molekulare Biophysik - Institut für Biologie, Invalidenstraße 42, 10115 Berlin, Germany*

*office: 214 --- phone: +49 30 2093 8466*

*email: **[log in to unmask]*

*www: **http://www2.hu-berlin.de/biologie/molbp/*